FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004980291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004980291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58856612
Sequences flagged as poor quality0
Sequence length27
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCG449700.019.8250561
CAGCGAG178700.019.554632
ATAGACC239350.019.4097331
TTGGCAA374950.019.375511
CGCGTGA146200.019.2749065
CAAGAAA461200.019.2282121
AAGAAAA190400.019.1970122
CCAGCGA336700.019.1365721
AGAAAAG191400.019.091493
CTGAAAC314400.019.0135861
CGCTGGA247450.019.007891
CGCGTCG153900.019.0015534
CGGCTAG343450.018.9745961
GATCGCG276000.018.9677621
TAGCGAG182300.018.8982723
AGGACGA114300.018.8857238
TGCGTGG312550.018.8773861
AGCTGAA224950.018.8561128
GCGTGGC260600.018.8501282
TGGCGAC129650.018.81212610
Produced by FastQC (version 0.11.5)