FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004980297

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004980297
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35787901
Sequences flagged as poor quality0
Sequence length27
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTGA274000.019.7349451
GCATGCG241050.019.4683111
TGCGCAA145150.018.9374754
CGCTGGA180450.018.9136711
ACTGATG136000.018.7829041
ACGAGCC136050.018.7219371
GGGACCT140050.018.6824111
CCACCTA199600.018.673141
TCTGAGC125650.018.6509594
GATCTAG216550.018.6430031
GAAGCTG114400.018.6036076
GCCGACA100500.018.5857183
GAAGACA80200.018.55096
CAATGGA90750.018.5463398
CTGATAG129050.018.5323681
CACCGAC105600.018.51343710
GGGCATC152400.018.4922831
CCGGTAG109400.018.432081
TGAGCCC107000.018.4085046
TACGGGC124300.018.3951971
Produced by FastQC (version 0.11.5)