FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004980320

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004980320
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20969883
Sequences flagged as poor quality0
Sequence length98
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN430930.20549947751258316No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT920550.073.546241
AGCAGTG976450.069.015282
CAGTGGT1256400.053.8078274
GTGGTAT1295800.053.6429026
AGTGGTA1314850.052.880895
GGTATCA1298400.051.461048
GCAGTGG1318350.051.362313
GTATCAA1314200.051.073229
TGGTATC1340300.049.9546057
CAACGCA1219850.029.6969912-13
ATCAACG1232450.029.64822810-11
GGTATAA99350.028.5803598
TACATGG1242100.028.42495322-23
GCAGAGT1307850.027.96941616-17
AGTACAT1294350.027.8536120-21
AGAGTAC1306100.027.7591118-19
TGGTATA101500.027.0696417
TCAACGC1211000.027.00526212-13
ACGCAGA1366900.026.62826314-15
GAGTACA1249650.026.1140720-21
Produced by FastQC (version 0.11.5)