FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004980326

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004980326
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20963097
Sequences flagged as poor quality0
Sequence length98
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN310830.1482748469846798No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT925200.073.9536061
AGCAGTG987050.068.880952
GTGGTAT1284500.054.58316
CAGTGGT1257400.054.20714
AGTGGTA1308750.053.603915
GGTATCA1284850.052.2130558
GTATCAA1300200.051.874889
GCAGTGG1330650.051.2849043
TGGTATC1322650.050.87657
GGTATAA101650.030.3319998
CAACGCA1211750.030.11054412-13
TGGTATA101200.030.0591267
ATCAACG1228600.029.93744510-11
TACATGG1235700.028.79438622-23
GCAGAGT1308300.028.34850916-17
AGAGTAC1297350.028.20398318-19
AGTACAT1289350.028.2031220-21
TCAACGC1204900.027.4290312-13
ACGCAGA1363300.027.04291514-15
GAGTACA1244450.026.52140820-21
Produced by FastQC (version 0.11.5)