FastQCFastQC Report
Tue 7 May 2024
EGAF00004980328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004980328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12662
Total Bases1.2 Mbp
Sequences flagged as poor quality0
Sequence length98
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG380.3001105670510188Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC340.2685199810456484Clontech SMARTer II A Oligonucleotide (100% over 25bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN210.1658505765281946No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCGCA180.14215763702416678Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGCGC170.1342599905228242Clontech SMARTer II A Oligonucleotide (100% over 23bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)