FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004980329

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004980329
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24825142
Sequences flagged as poor quality0
Sequence length27
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGGA175350.020.1102051
GAATAAG143850.019.6417981
CACCGAA149500.019.5101498
GCCACGA156450.019.5025446
CAATGGA137150.019.376088
GAGCAGA74600.019.3670791
GATGAAA123350.019.3369061
CGAGAAG111800.019.276321
GACTGAG101400.019.2595563
GGGATGC78900.019.1895482
CGGACTG115000.019.178511
TCGCGAC96200.019.15453310
TGTTCCG165750.019.0878721
GCTGCAG129500.019.083931
GACACGC178250.019.0402761
GATGCTA107650.018.9847931
GCACCGA87400.018.9568797
CTGATAG102150.018.9474851
TATGCCC122600.018.9066831
GTGAGCC69100.018.8872872
Produced by FastQC (version 0.11.5)