FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004980330

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004980330
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24825142
Sequences flagged as poor quality0
Sequence length98
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN383090.1543153308045529No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT952950.071.293051
AGCAGTG1065700.063.4149932
GCAGTGG1292850.052.4085163
CAGTGGT1294600.052.2451824
AGTGGTA1355900.050.9865155
GTGGTAT1392600.049.5384376
GGTATCA1419300.047.133628
GTATCAA1436800.046.7506039
TGGTATC1437650.046.6144037
ATCAACG1365150.026.98213610-11
CAACGCA1358200.026.90308412-13
AGAGTAC1442650.025.25303618-19
TACATGG1412200.025.21013622-23
AGTACAT1462050.024.79004120-21
GGTATAA94000.024.5807828
GCAGAGT1507300.024.5219816-17
TCAACGC1349950.024.40165512-13
ACGCAGA1543950.023.84309414-15
GAGTACA1393450.023.52730220-21
CGCAGAG1428750.023.10358416-17
Produced by FastQC (version 0.11.5)