FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004980343

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004980343
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29099451
Sequences flagged as poor quality0
Sequence length27
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCGGA100900.019.291571
ACGAGGA109650.019.2282641
TGCGTGG84500.018.9932841
CGGACTG108700.018.970871
TCGGCGC140350.018.9417938
GACTGCG112450.018.889681
GATCGCG106400.018.8080221
ATAGACC96650.018.7587971
CGGCGCA105950.018.7096169
GACTAAC114100.018.6533621
ATGGGAG124700.018.568031
CCGGTAG72750.018.5069081
GCCGAAG59150.018.4608673
CATGGCG103950.018.452541
GCGCGAT100050.018.4469761
CTCGAGG104050.018.41461
TGAGCAT129350.018.3474311
TCTGGAA100450.018.3002761
GGGATGA75850.018.2770861
TGACAAC100950.018.2721041
Produced by FastQC (version 0.11.5)