FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004980351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004980351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66466512
Sequences flagged as poor quality0
Sequence length27
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAGTA415400.019.9379734
ATGGGAG335600.019.5659561
AATTGGA429050.019.5258110
CTCAGAA491850.019.3886741
GAAGTAA435400.019.3476835
TGGCCAG487750.019.3275661
CCGAAGT449050.019.2716333
GCCAGAG429550.019.2078573
CCGGTAG378700.019.2068231
AAGAAGG246150.019.1944777
TAATTGG442100.018.9731929
CAGTGAA271250.018.9472687
GGGTTGC424050.018.8851261
ATAGAGA197050.018.884174
AACCGCG293900.018.873471
TGCGAGG189750.018.8470544
CAATGGA267350.018.8389668
GCGAGGG142850.018.7871655
CGCTGGA253250.018.7573281
CGGCTAG255350.018.7347721
Produced by FastQC (version 0.11.5)