FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004980402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004980402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25005463
Sequences flagged as poor quality0
Sequence length98
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN487610.19500138829662944No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT943100.071.5541461
AGCAGTG1070350.062.698942
GCAGTGG1288500.052.190863
CAGTGGT1294650.051.92164
AGTGGTA1366200.050.2673455
GTGGTAT1392850.049.218246
GGTATCA1413800.046.933678
GTATCAA1440650.046.2516989
TGGTATC1440400.046.211067
CAACGCA1357700.026.99944312-13
ATCAACG1365000.026.95495210-11
AGAGTAC1439900.025.41562818-19
TACATGG1422100.025.23534622-23
GGTATAA90300.025.0769658
AGTACAT1470550.024.72959520-21
GCAGAGT1505350.024.64726816-17
TCAACGC1352050.024.1937712-13
TGGTATA92850.024.0431047
ACGCAGA1546150.023.90657814-15
GAGTACA1392350.023.40189220-21
Produced by FastQC (version 0.11.5)