FastQCFastQC Report
Sat 4 Jun 2022
EGAF00004982242

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982242
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences253534763
Sequences flagged as poor quality0
Sequence length91
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT41646611.6426390411795326Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9244700.36463244292854624Clontech SMART CDS Primer II A (100% over 24bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4358930.17192632475413244No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3564280.14058348282598232Clontech SMART CDS Primer II A (100% over 25bp)
GTTTCTGGCTCATGTGGAGACCCATGAGCAGGGCTCCCACAATCACCACG3095000.12207398951440832No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)