FastQCFastQC Report
Fri 17 May 2024
EGAF00004982250

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982250
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences260029045
Total Bases23.6 Gbp
Sequences flagged as poor quality0
Sequence length91
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT47472541.8256629754572224Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11004760.4232127222557003Clontech SMART CDS Primer II A (100% over 24bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7126040.27404784723183523No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3964350.1524579686857674Clontech SMART CDS Primer II A (100% over 25bp)
GTTTCTGGCTCATGTGGAGACCCATGAGCAGGGCTCCCACAATCACCACG3566100.1371423719223366No Hit
GTGGTAACCAGGTGCTCACTCAGGGCCAGGCGTTGCTGGGCTTCCGGCGC2718110.10453101498719114No Hit
GTGGAGACCCATGAGCAGGGCTCCCACAATCACCACGACGATGAGGACCA2685430.10327423230739473No Hit
GCTTCCGGCGCCCCAATGCTCATCTCCAGAACCATCTCCGTGTGTTTCTG2665410.10250431831567125No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)