FastQCFastQC Report
Sat 4 Jun 2022
EGAF00004982252

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982252
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences259917859
Sequences flagged as poor quality0
Sequence length91
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT47377981.822805873450966Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10994580.4230020992901454Clontech SMART CDS Primer II A (100% over 24bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7212120.2774768931903213No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3948180.15190106655964722Clontech SMART CDS Primer II A (100% over 25bp)
GTTTCTGGCTCATGTGGAGACCCATGAGCAGGGCTCCCACAATCACCACG3559540.13694865038111906No Hit
GTGGTAACCAGGTGCTCACTCAGGGCCAGGCGTTGCTGGGCTTCCGGCGC2727130.10492276331038877No Hit
GTGGAGACCCATGAGCAGGGCTCCCACAATCACCACGACGATGAGGACCA2681870.1031814439499519No Hit
GCTTCCGGCGCCCCAATGCTCATCTCCAGAACCATCTCCGTGTGTTTCTG2660430.1023565679648046No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)