FastQCFastQC Report
Sun 19 May 2024
EGAF00004982258

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982258
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences246841337
Total Bases22.4 Gbp
Sequences flagged as poor quality0
Sequence length91
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT42978211.7411269328848271Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8774580.35547449655889685Clontech SMART CDS Primer II A (100% over 24bp)
CCCAGCTCAGCCACACACTTCTGGGAGCCCTCTATGAGGTGGTTCACGGG4691710.19006986662043562No Hit
GCCCAGGCTGCTCAGCAGGAGCTTCAGCGGGTTGAGGGTGCCGAGGGGGT3779060.15309672382790568No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3692650.14959609459577672Clontech SMART CDS Primer II A (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3551740.14388756936606611No Hit
GGTGGTTCACGGGGATGCCCAGGCTGCTCAGCAGGAGCTTCAGCGGGTTG3148190.12753901102067033No Hit
GCCACACACTTCTGGGAGCCCTCTATGAGGTGGTTCACGGGGATGCCCAG3083500.12491829923932068No Hit
CTTCTGGGAGCCCTCTATGAGGTGGTTCACGGGGATGCCCAGGCTGCTCA2972500.12042148353782413No Hit
GGTTCACGGGGATGCCCAGGCTGCTCAGCAGGAGCTTCAGCGGGTTGAGG2950780.11954156608704482No Hit
CTCAGCAGGAGCTTCAGCGGGTTGAGGGTGCCGAGGGGGTTGGCCAGGGT2882430.11677258092310527No Hit
GCCCTCTATGAGGTGGTTCACGGGGATGCCCAGGCTGCTCAGCAGGAGCT2600430.10534823833011406No Hit
GCTTCAGCGGGTTGAGGGTGCCGAGGGGGTTGGCCAGGGTCCCGGCCCCG2572000.1041964863445866No Hit
AGCGGGTTGAGGGTGCCGAGGGGGTTGGCCAGGGTCCCGGCCCCGGCCTC2474320.10023928852726964No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)