FastQCFastQC Report
Sat 4 Jun 2022
EGAF00004982260

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982260
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences246850841
Sequences flagged as poor quality0
Sequence length91
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT43048801.7439195193991661Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8803690.35664006508286517Clontech SMART CDS Primer II A (100% over 24bp)
CCCAGCTCAGCCACACACTTCTGGGAGCCCTCTATGAGGTGGTTCACGGG4716750.19107692649100597No Hit
GCCCAGGCTGCTCAGCAGGAGCTTCAGCGGGTTGAGGGTGCCGAGGGGGT3777460.15302601298409188No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3678550.1490191398618731Clontech SMART CDS Primer II A (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3504110.14195252427760616No Hit
GGTGGTTCACGGGGATGCCCAGGCTGCTCAGCAGGAGCTTCAGCGGGTTG3141810.12727564497136956No Hit
GCCACACACTTCTGGGAGCCCTCTATGAGGTGGTTCACGGGGATGCCCAG3077420.12466718717802545No Hit
CTTCTGGGAGCCCTCTATGAGGTGGTTCACGGGGATGCCCAGGCTGCTCA2960890.119946522685738No Hit
GGTTCACGGGGATGCCCAGGCTGCTCAGCAGGAGCTTCAGCGGGTTGAGG2942070.11918411896356472No Hit
CTCAGCAGGAGCTTCAGCGGGTTGAGGGTGCCGAGGGGGTTGGCCAGGGT2882290.11676241362288896No Hit
GCCCTCTATGAGGTGGTTCACGGGGATGCCCAGGCTGCTCAGCAGGAGCT2600640.10535268948101335No Hit
GCTTCAGCGGGTTGAGGGTGCCGAGGGGGTTGGCCAGGGTCCCGGCCCCG2558870.10366057452484029No Hit
AGCGGGTTGAGGGTGCCGAGGGGGTTGGCCAGGGTCCCGGCCCCGGCCTC2480820.10049874612337253No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)