FastQCFastQC Report
Fri 27 May 2022
EGAF00004982266

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982266
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences271853815
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT143550195.280418448422362Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT23521300.8652186837988645Clontech SMART CDS Primer II A (100% over 24bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12659250.4656638715921643Clontech SMART CDS Primer II A (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9197710.3383329382374126No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4153050.15276776601424555Clontech SMART CDS Primer II A (100% over 23bp)
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3369070.123929472904399Clontech SMART CDS Primer II A (100% over 22bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)