FastQCFastQC Report
Sat 11 May 2024
EGAF00004982322

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982322
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28439393
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15116895.315475615108944Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3078861.0826039782213355Clontech SMART CDS Primer II A (100% over 24bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1576640.5543859533148263Clontech SMART CDS Primer II A (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG816730.2871826413454042No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT600670.21121055572458947Clontech SMART CDS Primer II A (100% over 23bp)
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT530480.18653000083370275Clontech SMART CDS Primer II A (100% over 22bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT439740.15462355332267463Clontech SMART CDS Primer II A (100% over 21bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)