FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982322

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982322
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28439393
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15116895.315475615108944No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3078861.0826039782213355No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1576640.5543859533148263No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG816730.2871826413454042No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT600670.21121055572458947No Hit
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT530480.18653000083370275No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT439740.15462355332267463No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1780850.079.3580861
AGCAGTG2002050.070.602562
TGGTATC2519350.056.0274057
GTATCAA2540300.055.639349
GGTATCA2544350.055.5269978
GTGGTAT2547850.055.427196
CAGTGGT2800800.050.5441784
GCAGTGG2842450.049.9156843
AGTGGTA2894900.048.896815
ATCAACG2527100.031.0013910-11
CAACGCA2549250.030.74372712-13
ACGCAGA2572000.030.46612514-15
AGAGTAC2568100.030.33111818-19
GCAGAGT2620600.029.86441816-17
AGTACTT2633700.029.36369920-21
TACTTTT2704800.028.55119922-23
TCAACGC2539800.027.86793312-13
TATCAAC2578350.027.4579110-11
AACGCAG2579450.027.44931414-15
CGCAGAG2608350.027.13277216-17
Produced by FastQC (version 0.11.5)