FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26751808
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14196315.306673104113187No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2864031.0705930604765106No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1476640.5519776457725772No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG757600.2831958124101369No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT563840.21076706292150424No Hit
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT502760.18793496125570278No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT411310.15375035586379807No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1674000.079.48841
AGCAGTG1880400.070.756012
TGGTATC2363950.056.232347
GTATCAA2383800.055.7955449
GGTATCA2386350.055.7187548
GTGGTAT2394750.055.5589036
CAGTGGT2623950.050.835234
GCAGTGG2667600.050.089433
AGTGGTA2715450.049.1144645
ATCAACG2375000.031.06864410-11
CAACGCA2391150.030.86318812-13
ACGCAGA2412050.030.60279814-15
AGAGTAC2414150.030.43106718-19
GCAGAGT2458450.030.0012616-17
AGTACTT2473550.029.5099920-21
TACTTTT2547600.028.61221522-23
TCAACGC2387150.027.9075212-13
AACGCAG2418850.027.5546914-15
TATCAAC2419900.027.53076210-11
CGCAGAG2443850.027.26964416-17
Produced by FastQC (version 0.11.5)