FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982337

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982337
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78935510
Sequences flagged as poor quality0
Sequence length26
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCCGCAGGCTCCACTCCTGGTGGT1234780.15642896333982007No Hit
CGTTCGTTATCGGAATTAACCAGACA1078050.1365735142523308No Hit
GTCTCGTTCGTTATCGGAATTAACCA1044430.13231434116280494No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGGGT257650.017.4042991
TGGCTGG379650.017.3261051
GGGACCT219200.017.3131031
CGTTGGG350350.017.2832831
GGCGACT202200.017.1612361
CGGACTG201150.017.136111
TGAGCCG272400.017.1026821
CGACCTT298700.017.0745581
TGGGCGT179600.016.9133131
CAATGAA206850.016.8662918
GGTATTG168350.016.7981361
CCCTCCT615650.016.7818681
GCCTCTA545150.016.7549481
ATCGAGT165350.016.7267551
GACCTGG266900.016.6545261
TGCCCTA392900.016.5988241
GGGAGAT162950.016.5667951
CAATGGA369150.016.5295628
CTGCCTA271400.016.4966661
GGCTCGA208550.016.4943961
Produced by FastQC (version 0.11.5)