FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982339

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982339
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74134687
Sequences flagged as poor quality0
Sequence length26
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCCGCAGGCTCCACTCCTGGTGGT1155160.15581909720614318No Hit
CGTTCGTTATCGGAATTAACCAGACA1003580.13537252811224523No Hit
GTCTCGTTCGTTATCGGAATTAACCA976750.13175343952015336No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGGGT241250.017.5642431
TGGCTGG358000.017.39421
TGAGCCG250450.017.3517321
GGGACCT211450.017.216121
GGCGACT187250.017.2065641
CGTTGGG334050.017.1394141
CGACCTT270700.017.0471121
CAATGAA192500.016.9132338
CGGACTG188350.016.813071
TGGGCGT161100.016.8105931
CCCTCCT579650.016.7409931
GGCTCGA191550.016.7102971
TGCCCTA364150.016.6788481
GCCTCTA502700.016.6588551
CTGCCTA259550.016.5811
GACCTGG241700.016.5434991
ATCGAGT156750.016.5409561
GGTATTG159900.016.5225871
ATGCGAT117750.016.4975761
CCGGGAT150800.016.4816381
Produced by FastQC (version 0.11.5)