FastQCFastQC Report
Fri 17 May 2024
EGAF00004982366

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982366
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences274795386
Total Bases25 Gbp
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT91239723.3202784562037735Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT25708130.9355371781970167Clontech SMART CDS Primer II A (100% over 24bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17465310.6355750820357661No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT7913830.2879899155220896Clontech SMART CDS Primer II A (100% over 25bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5966470.21712409683618197Clontech SMART CDS Primer II A (100% over 21bp)
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4081330.14852250830732652Clontech SMART CDS Primer II A (100% over 22bp)
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3217930.11710276678371886Clontech SMART CDS Primer II A (100% over 23bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)