FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20141469
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA461940.22934772036736747No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT293220.14558024541308284No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC290480.14421986797487313No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAA238730.11852660796489074No Hit
GGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCG220650.10955010282517129No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC211450.10498241215672999No Hit
CTAAGATCAAGTGTAGTATCTGTTCTTATCAGTTTAATATCTGATACGTC206170.10236095490353757No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT169400.035.053176
CGTACGG14650.033.709373
AGACCGC178100.033.2072264
CCGCGTT185050.032.088667
CGCGTTC185550.031.905998
GGTCGAC67700.030.1936651
GCGTTCT197250.030.0737979
GACCGCG200150.029.7569625
CAGACCG219050.027.2166463
TCGACCC82250.025.3910883
GTCGACC83150.024.6869222
CTCAGAC285250.022.6889571
TCAGACC276100.022.131672
CTCGCTA258600.021.4896097
ACCCGTG98800.021.318496
CCACTCA152500.021.2588331
GTCTCGC269950.021.115065
GGCGTAC25350.020.9229281
TGGCGCG117800.020.4054532
TGCGACA41750.020.0942946
Produced by FastQC (version 0.11.5)