FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20141469
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA353380.17544897047975994No Hit
CTAAGATCAAGTGTAGTATCTGTTCTTATCAGTTTAATATCTGATACGTC267310.1327162383240269No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT246810.12253823194326094No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC215530.10700808367056047No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT202960.10076722805074445No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT130750.034.856786
AGACCGC139600.032.5184334
CGCGTTC144250.031.5532198
CCGCGTT145800.031.2177757
GACCGCG157200.029.0669295
GGTCGAC66800.028.5903221
GCGTTCT160700.028.1752579
TGTCTCG49300.028.1189754
CAGACCG180900.025.5547643
CGTACGG13400.024.8640943
TGGCGCG111350.024.0442752
CTAAGAT177900.023.4106031
TAAGATC173600.023.0993422
GTCGACC85150.022.638612
TCGACCC84850.022.5082953
GTCTCGC240350.022.3529135
TGCGACA49700.022.2667456
GCGACAT50900.021.9754687
CGGCCTT111850.021.3835871
CTCGCTA229500.021.3620327
Produced by FastQC (version 0.11.5)