FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982465

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982465
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19409410
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1102740.5681470997830433No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA821340.42316587675771705No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC695630.35839832328751875No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT597270.30772187305023696No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC538980.2776900482807051No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA443060.22827072023312406No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG410770.21163445978007572No Hit
CCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTC398580.20535400097169365No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC379490.19551856547932162No Hit
CACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCA317280.16346710178207374No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG256430.13211632914138038No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAA248720.12814402910753084No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA248630.1280976598464353No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA234530.12083314227480382No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG197920.10197115728917056No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCTA200850.052.511391
TCGCTAT204200.051.608832
ACCGCGT171500.047.2450336
CGCTATG224700.047.0777243
AGACCGC172250.047.017934
CGCGTTC176100.046.0378958
CCGCGTT182100.044.5731547
GCGTTCT191550.042.5725259
CAGACCG206750.039.3789023
GCTATGT278800.038.5047234
TATGTTG316750.035.1465156
CTATGTT318800.033.852145
TGGAGTC337150.033.2606432
GAGTCTT336450.033.248244
CTCAGAC294350.033.047561
TCAGACC252450.032.8534552
ATGTTGC344650.032.6458557
GGAGTCT343050.032.5670363
AGTCTTG358700.031.3711015
GTCTTGG366450.030.9370566
Produced by FastQC (version 0.11.5)