FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982498

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982498
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17053538
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG511630.3000139912316142Illumina Single End PCR Primer 1 (100% over 50bp)
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT400270.23471375851744075No Hit
CGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGT290270.1702110142775065No Hit
CGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGT259170.1519743293151251No Hit
GGCCTTTTGGCTAAGATCAAGTGTAGTATCTGTTCTTATCAGTTTAATAT239370.14036383535193694No Hit
CAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAG230000.13486937431986254No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN223240.1309053874920266No Hit
GGGAAGCTCATCAGTGGGGCCACGAGCTGAGTGCGTCCTGTCACTCCACT213640.12527605708563233No Hit
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTT207500.12167563117987598No Hit
CGGCCTTTTGGCTAAGATCAAGTGTAGTATCTGTTCTTATCAGTTTAATA200820.11775855543875997No Hit
GGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGA195240.11448650714004333No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC193480.11345446323220436No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG191710.11241655543852541No Hit
GGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCA186740.10950220417604839No Hit
CCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCA175830.10310470472461493No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA175060.10265318551493537No Hit
GGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGAT173770.10189674424157616No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG173360.10165632492213639No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA73800.042.9039346-47
CGTATCA78200.040.5277748-49
GGGCGAT51400.034.7269671
GGCGATC52300.033.7883762
TGGTCGC101400.031.8408342-43
GCCGTAT101800.031.5709646-47
GGTCGAC53800.030.7496627
AGGTCGA54200.030.7423156
CCGTATC104200.030.50095648-49
TCGCCGT105000.030.24035844-45
CGATCTG64600.028.3718934
GGTCGCC115200.028.1815542-43
GTGGTCG115400.028.05386240-41
GCGATCT65400.027.9312973
TCTCGGT116200.027.8377536-37
GTATCAT121600.026.38076650-51
GATCTCG123100.026.36495634-35
TCGGTGG124300.026.03919638-39
CGGCCTT94900.025.3920351
GGCTAAG129650.024.3236479
Produced by FastQC (version 0.11.5)