FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982503

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982503
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18511677
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAA477860.2581397676720483No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA459170.24804343766369735No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC412430.22279450964923384No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT271580.14670739987522471No Hit
GCGGAGGAGAGTAGTCTGAATTGGGTTATGAGGTCCCCTGCGGGGTACCT252890.13661106986687374No Hit
GGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCG218630.11810383251609241No Hit
CGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGC204890.11068149039117309No Hit
ACGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGG203270.10980636708386819No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCTG61550.030.1566163
CCGCGCT63750.028.2760332
ACCGCGT235650.028.1490359
AGACCGC238100.027.8094147
GCACCGT65150.027.6245541
CAGACCG244050.027.0826596
CCACTCA230300.026.601241
GCGCTGA74600.025.9976944
GACCGCG264850.025.0904968
CTCGCTA307950.023.336888
GTCTCGC314150.023.3308036
GAGTAGT147200.023.117689
TCGCTAT315300.022.8683439
CCGCGTT261250.022.7719277
GGTCTCG326100.022.3298955
CGCGTTC276050.022.13292310-11
ACTCAGA294450.022.1237433
CACCGTT83350.021.9837192
GGGGTCT341750.021.6728943
TCTCGCT346450.021.3617047
Produced by FastQC (version 0.11.5)