FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982504

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982504
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18511677
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAAGCTCATCAGTGGGGCCACGAGCTGAGTGCGTCCTGTCACTCCACT328300.17734751962234432No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGA229120.12377052603067783No Hit
TTTCAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGT223310.12063196651497324No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT222020.11993510906656377No Hit
GGCCTTTTGGCTAAGATCAAGTGTAGTATCTGTTCTTATCAGTTTAATAT190250.10277296865108439No Hit
CAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTA186210.10059056237854626No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAT47600.044.254971
GGCGATC50400.042.0324972
CGGCCTT66650.030.8916931
GCGATCT81600.026.544653
CGATCTG81200.026.0891384
AGGTCGA42300.021.805056
GCCACGA141000.020.81854818-19
GCTCATC213700.020.494586
GGTCGAC44150.020.4870137
GGCTAAG134100.019.8321179
GGGAAGC235450.019.6375851
GGCGCGT136850.019.1325841
TGCGACA85850.018.74545712-13
CATCAGT236350.018.6028339
GAAGCTC237200.018.5393923
AGCTCAT236650.018.4818975
CACGAGC158900.018.45631220-21
AAGCTCA243950.018.0508084
GCGACAT88800.018.0262377
CTCATCA251550.017.718357
Produced by FastQC (version 0.11.5)