FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982508

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982508
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30528190
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT25250.024.076542
CGTATGC25550.023.88427744
TCGTATG25500.023.66510443
TCTCGTA27900.021.37746241
GATCGGA30850.019.9852161
ATCGGAA32800.019.0010092
ATCTCGT34150.017.79139340
GTATGCC37250.016.26258745
ACACGTC39900.015.2235813
CGTCTGA46000.013.84042916
ACGTCTG48450.012.95482115
TCGGAAG53950.012.2187573
CACGTCT53800.012.1682914
CACACGT53750.011.5100912
CGGAAGA67300.09.8949914
GCACACG74350.08.77491611
GTCACAC76500.08.705090529
TCTGAAC80500.08.24472718
GATCTCG91900.07.247345439
AGAGCAC93600.07.210678
Produced by FastQC (version 0.11.5)