FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11484245
Sequences flagged as poor quality0
Sequence length51
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC1536661.3380592280990173TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG165850.042.86900343
CTCGTAT166050.042.7631742
TATCTCG167600.042.45814539
CGTATGC167300.042.4571144
TCTCGTA167950.042.30618741
GTATGCC169050.042.01759345
ATCTCGT170200.041.7302440
ATGTATC174000.040.8445736
GATGTAT174750.040.72076835
GTATCTC175150.040.58923338
TGTATCT178650.039.83181837
CGATGTA182050.039.1620634
CCGATGT186950.038.17171533
ACCGATG191050.037.43496332
CACCGAT196100.036.5053531
TCACCGA211250.033.9192830
ATCGGAA219300.033.497752
GATCGGA222050.033.1786421
ACACGTC219100.033.15595213
CGTCTGA220100.032.92339316