FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30959829
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC426130.13763964910788107TruSeq Adapter, Index 3 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG54150.037.67653743
CTCGTAT54900.037.2426342
CGTATGC55400.036.66396744
TCTCGTA60200.033.8890741
ATCGGAA65750.031.6722952
GATCGGA66650.031.10531
ATCTCGT65950.030.89946740
ACACGTC71350.029.19024713
GTATGCC70300.029.14254645
CGTCTGA77200.026.92063316
ACGTCTG82600.025.24237815
CACACGT86750.024.11205512
CATCTCG86500.023.79259939
CACGTCT89150.023.53862414
TCGGAAG93650.022.5014923
ACTTAGG94300.022.20662932
TAGGCAT94650.021.91084535
TTAGGCA98750.021.34255634
CTTAGGC99200.021.3137633
CGGAAGA103550.020.5025714
Produced by FastQC (version 0.11.5)