FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982512

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982512
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19148319
Sequences flagged as poor quality0
Sequence length51
%GC62

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC582080.3039849085447135TruSeq Adapter, Index 10 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG65750.041.7141243
CTCGTAT68000.040.30068242
CGTATGC68500.040.16843844
TCTCGTA71050.038.69713641
TATCTCG71550.038.3323839
TAGCTTA71450.038.25857534
GTATGCC72850.037.80079345
ATCTCGT73550.037.41239540
ATCGGAA74950.036.7699552
AGCTTAT75400.036.34382635
GCTTATC76650.035.8391936
GTCACTA77500.035.38630329
GATCGGA78850.035.1343041
ACTAGCT79250.034.8621232
ACACGTC81000.034.301313
TCACTAG80800.034.10812830
ACGTCTG85300.032.70403315
CACTAGC85000.032.66261731
CGTCTGA85850.032.41589716
CTAGCTT85800.032.1220733
Produced by FastQC (version 0.11.5)