FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982513

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982513
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19224410
Sequences flagged as poor quality0
Sequence length51
%GC62

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC586880.3052785495107522TruSeq Adapter, Index 10 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG66950.041.2327643
CTCGTAT68150.040.53973842
CGTATGC68400.040.32578344
TCTCGTA70950.039.03499241
TATCTCG71050.038.9167239
GTATGCC73400.037.76270345
ATCGGAA74650.037.7393232
TAGCTTA74350.037.21967334
AGCTTAT75250.036.8343235
ATCTCGT75450.036.766540
GCTTATC76850.036.21381436
GATCGGA78050.036.211381
TCACTAG79700.035.25758430
ACTAGCT79900.034.97222532
GTCACTA79950.034.9222129
ACACGTC82850.034.18862513
CACTAGC84700.033.0434531
CGTCTGA86500.032.69396616
ACGTCTG87300.032.42013515
CTAGCTT86250.032.39745733
Produced by FastQC (version 0.11.5)