FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19335974
Sequences flagged as poor quality0
Sequence length51
%GC61

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC22800.033.8469644
TCGTATG23650.032.82107543
CTCGTAT24250.032.10203642
TCTCGTA26150.030.28580941
TATCTCG27250.029.64214539
ATCGGAA32600.028.3659232
GTATGCC28650.026.93586745
ATCTCGT29950.026.89470540
CGCCAAT32400.026.80758333
GATCGGA36350.025.465931
CAATATC34450.024.29565636
ACACGTC39700.023.68896713
ATATCTC37550.022.41007638
CCAATAT39250.021.95605535
AGTCACG44100.021.0716628
CGTCTGA44450.020.90417516
ACGTCTG45750.020.45791815
GCCAATA42300.020.4269234
ACGCCAA44650.019.95666532
CACACGT48050.019.29131912
Produced by FastQC (version 0.11.5)