FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982518

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982518
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27216754
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC877540.3224263995625636TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC107100.039.58117744
TCGTATG107850.039.30592343
CTCGTAT108750.038.85642642
TCTCGTA109000.038.0444241
ATCGGAA116800.037.0679242
GATCGGA117100.036.9874841
GTATGCC114800.036.98483345
TCACGAT113900.036.79506735
ATCTCGT115850.035.8532240
ACACGTC122350.035.2941613
ACGATCT118200.035.19553837
CATCACG124850.034.22981633
ATCACGA125950.033.89974234
CGTCTGA128600.033.4214516
ACGTCTG128850.033.3391515
CACGATC126250.033.0177336
CACACGT132150.032.6256912
CACGTCT135800.031.76540614
GTCACAT139000.030.73882929
TCGGAAG143850.030.3168933
Produced by FastQC (version 0.11.5)