FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982520

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982520
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23880874
Sequences flagged as poor quality0
Sequence length51
%GC60

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC249920.10465278615849655TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT39000.033.1741142
TATCTCG39550.032.8223739
TCGTATG39450.032.7387343
CGTATGC39700.032.41928544
TCTCGTA41100.031.4757241
ATCGGAA45150.029.2994962
CGCCAAT45100.028.83881233
GTATGCC44900.028.56448645
ATCTCGT45900.028.08552440
GATCGGA48300.027.1887781
ACACGTC50750.026.5140313
CAATATC50550.025.85792736
AGTCACG52500.025.24475328
ATATCTC53200.024.6968138
ACGTCTG56850.023.74415815
GCCAATA56400.023.7390834
CCAATAT56300.023.50112335
CGTCTGA59850.022.36602616
CACACGT61350.021.89632612
CACGTCT62100.021.80925814
Produced by FastQC (version 0.11.5)