FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982522

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982522
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12841290
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC6867285.347811629516972TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA703050.044.2246241
CTCGTAT702800.044.2147442
CGTATGC703650.044.17732244
TCGTATG704200.044.13961843
ATCTCGT705250.044.09513540
CATCTCG710400.043.85780339
CGTCTGA718700.043.7958116
ACACGTC720450.043.7489313
GTATGCC710500.043.74823845
ACGTCTG722100.043.5991115
CACGTCT724750.043.47073714
GATCGGA729400.043.446621
ATCGGAA729300.043.4298022
TCGGAAG731000.043.3041733
CGGAAGA731250.043.2587554
CACACGT729850.043.20396812
GCACACG733750.043.0448511
GATCATC725100.042.9748836
ACCAGAT734050.042.63802732
CCAGATC733550.042.6026833
Produced by FastQC (version 0.11.5)