FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004982524

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25166348
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC10501144.1726912462626675TruSeq Adapter, Index 3 (100% over 51bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGG761890.3027415817344654TruSeq Adapter, Index 3 (100% over 38bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT584230.23214731036859224Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA508090.20189262264036084TruSeq Adapter, Index 3 (100% over 36bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA400330.15907353740797037Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCA379150.15065753680271768TruSeq Adapter, Index 3 (100% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)