FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30455579
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATG1041630.3420161540846096TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA118150.042.055931
CTCGTAT122150.040.0603144
TCGTATG123050.039.82215545
ATCGGAA124900.039.7907182
TCTCGTA123700.039.50377743
TATCTCG126600.038.63536541
GTCACGT133150.037.10598429
TCGGAAG135250.036.8122753
ATCTCGT133000.036.79313742
ACACGTC135350.036.5523613
AGTCACG136450.036.24150528
CGGAAGA138000.036.013484
CGTCTGA138650.035.7472916
ACGTCTG146850.033.79714615
TCACGTG152700.032.45848530
CACGTCT153550.032.3517514
ACGTGGC156250.031.72123532
CACACGT158300.031.46626512
GCCTTAT160000.030.7674537
CGTGGCC163000.030.50424833
Produced by FastQC (version 0.11.5)