FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982530

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982530
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20472563
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG4119692.012298118218027TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA439450.044.495031
ATCGGAA441400.044.284572
TCGGAAG445900.043.8642043
TCGTATG439600.043.79282445
TCTCGTA443050.043.63942343
CTCGTAT442650.043.62801744
ACACGTC455850.043.61361313
CGTCTGA457850.043.3450116
CGGAAGA458050.043.3390434
ATCTCGT446950.043.32334542
ACGTCTG461800.043.0765615
CACGTCT465750.042.73485614
CACACGT471100.042.25483312
GCACACG473400.042.07825511
AATCTCG469600.041.56438441
ACATGTC483750.040.6415332
TCTGAAC490650.040.4473918
GAATCTC491750.039.74115840
GTCACAT495250.039.7392729
AGAGCAC501150.039.7303168
Produced by FastQC (version 0.11.5)