FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982531

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982531
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20515761
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG4177092.0360395112811074TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA439800.044.5639761
ATCGGAA441550.044.3940052
TCGGAAG447300.043.853243
ACACGTC450750.043.82925813
CTCGTAT442400.043.7842444
TCTCGTA443050.043.7657243
TCGTATG441500.043.76106645
CGGAAGA454600.043.499714
CGTCTGA455650.043.29437316
ATCTCGT449650.043.14771742
ACGTCTG458350.043.0982415
CACGTCT463650.042.6098114
CACACGT467550.042.28325712
GCACACG471850.041.97390411
AATCTCG470800.041.45315641
ACATGTC483700.040.54918732
TCTGAAC489150.040.343718
AGAGCAC493900.040.0777058
GAATCTC490250.039.86568540
GTCACAT492750.039.82271229
Produced by FastQC (version 0.11.5)