FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32955483
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC3072820.9324154041377576TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA328100.044.0917361
ATCGGAA335550.043.1130372
CTCGTAT334450.042.4800542
CGTATGC335600.042.45548244
TCGTATG336450.042.36804243
TCTCGTA329750.042.3345541
TCGGAAG343500.042.148353
ACACGTC343450.042.03526713
CGGAAGA344600.041.9746484
CGTCTGA344500.041.8613816
ATCTCGT336250.041.5094940
ACGTCTG355700.040.56231715
CATCTCG346400.040.32533339
CACGTCT360050.040.0847514
CACACGT364350.039.63013512
GCACACG366700.039.43136611
GTATGCC365850.039.10448545
GATCATC378700.036.99693736
CCAGATC398800.036.05864333
ACCAGAT403750.035.6227932
Produced by FastQC (version 0.11.5)