FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24115513
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA9050.017.4243531
CGTCCCG9650.015.15805635
CTCGTAT10600.013.79563144
ATCGGAA12300.013.7177272
TCGTATG12000.012.56109945
TCTCGTA10500.012.21292543
TCCCGAT12250.012.12099737
CCCGATC12100.012.08520438
CCGATCT13150.011.46238739
GTCCCGA14000.010.76672436
TCGGAAG16350.010.7325483
ACACGTC16850.09.61394913
ACCCGTC18350.08.708055532
ATCTCGT17100.08.15696842
CGGAAGA21900.07.80720334
CACCCGT20400.07.72265831
TCACCCG23750.07.2018730
CGTCTGA24800.06.985021616
CCGTCCC23150.06.902526434
ACGTCTG25800.06.45268915
Produced by FastQC (version 0.11.5)