FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23977284
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA8850.017.5612051
CGTCCCG9300.015.2438535
CTCGTAT10150.013.96552344
ATCGGAA11350.013.6769652
TCTCGTA11250.012.80000543
TCGTATG12000.011.81250545
TCCCGAT12100.011.71361137
CCCGATC12150.011.29521738
TCGGAAG16100.010.3404983
CCGATCT13100.010.3043839
ACACGTC17150.09.70894413
GTCCCGA15200.09.02874336
ACCCGTC17700.08.77239832
ATCTCGT17850.08.1932842
CACCCGT19350.08.02436431
CGGAAGA21500.07.743354
TCACCCG23900.07.720731730
CCGTCCC25000.07.020969434
CGTCTGA28050.06.49662416
ACGTCTG27950.06.117432615
Produced by FastQC (version 0.11.5)