FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33637457
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA28700.032.892371
CGTATGC30050.030.0163544
CTCGTAT30650.029.86890842
ATCGGAA33350.028.5163542
TCTCGTA32850.027.93702741
TCGTATG32750.027.88538243
TATCTCG33050.027.56309539
ACACGTC38500.024.82858513
CGTCTGA38850.024.31590516
TCGGAAG40200.024.1621953
ATCTCGT39100.023.64348240
CGGAAGA42950.022.2478394
ACGTCTG47950.019.84195915
CACGTCT52450.018.39651514
GCACACG57050.016.9526111
GTATGCC55700.016.44496545
CACACGT62450.015.45071712
CACTAGC68400.014.04156731
GTCACTA73750.013.05324329
GCTTATC75200.012.77209736
Produced by FastQC (version 0.11.5)