FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18137859
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC3133261.7274695982585377TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA346350.044.498311
ATCGGAA348400.044.094752
CTCGTAT346200.043.8851342
TCTCGTA347750.043.7024641
CGTATGC347450.043.6560244
TCGTATG348800.043.5902643
ACACGTC351950.043.55809813
TCGGAAG353250.043.4614833
CGTCTGA353800.043.3366916
ATCTCGT349750.043.25314340
CGGAAGA357150.043.012094
GTATGCC355350.042.74246245
ACGTCTG359100.042.74093615
CACGTCT364000.042.1841214
CACACGT367300.041.8051212
GCACACG369800.041.57116711
AATCTCG366300.041.21291439
GTAATCT375050.040.19742237
CCTTGTA382150.039.90979433
TCTGAAC385400.039.8009118
Produced by FastQC (version 0.11.5)