FastQCFastQC Report
Wed 20 Apr 2022
EGAF00004982544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13431519
Sequences flagged as poor quality0
Sequence length125
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCGG251.7275754E-595.186865
CGAGTCG3000.065.440968
GCGCGTT701.3369572E-859.535221
ACGCGTC709.157302E-750.9929588
CGCGCTA952.6229827E-950.1349261
CGGGCGT952.6229827E-950.1349261
CGGCCCG952.6393536E-950.0983473
CGACGCG255.337597E-447.594845108-109
TCCGCCG1152.764864E-1046.558799
CGGGTCG355.4553147E-542.49990530-31
CCGCGGT659.1858965E-1041.187847108-109
CCGCGAC600.001309899839.6611943
AGCCGCG758.54925E-1139.66118694-95
CCCCGCG650.001940667936.611422
GGCGCCG650.001940951736.610337
CTCGCGC901.4551915E-1136.3560994-95
GTCGCGT501.063007E-535.70031768-69
CCGGCAT6200.034.568841
CCGCGCA350.00278167934.0209554-55
CGCCCGG1451.0935946E-732.847021
Produced by FastQC (version 0.11.5)