FastQCFastQC Report
Mon 18 Apr 2022
EGAF00004982552

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982552
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42750013
Sequences flagged as poor quality0
Sequence length125
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT655330.15329352063588847No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG16050.044.8427355
CGGGCGT5000.042.8629071
GCGGGTT24950.038.4153251
ACGCGCG951.1730644E-637.567258
GCGCGTT5300.037.0669821
CGCGTAT7800.036.6349641
ACCGCGT22700.031.4439536
CGCGGTT7050.031.2436521
CCGCGTT22900.031.1693347
CGCGTTC23600.030.496868
CGCGGAT8450.030.29431
GCCGGAT21400.029.7659111
CGAGTCG7700.029.3544968
CGGCGTT6700.029.2978762
GCGGGTA24250.028.2315831
CGGGTCG2853.6379788E-1227.1548651
CGCGAAT5500.027.059921
TCGCGCC16500.027.037174-75
CGCGCTA3100.026.8853361
CGGGCGG3100.026.8853361
Produced by FastQC (version 0.11.5)