FastQCFastQC Report
Mon 18 Apr 2022
EGAF00004982553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42750013
Sequences flagged as poor quality0
Sequence length125
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGCG1455.820766E-1141.054011
CGCGCCC3750.030.161012
CGCGGGC1306.362937E-527.4746063
ACGCGCG1303.05281E-822.88396574-75
CGCGCCG2102.7825263E-622.6774521
CGCACGC4750.022.5512897
GGCGCGA17150.020.98919930-31
CGGTCGC2055.939196E-520.3189438
CGCGACC2500.020.229424100-101
CGTAGCC32100.020.0205338
GCGCCCG4458.0035534E-1118.7280083
TCGGTCG3104.140742E-617.2771977
CGACCGT7400.016.8866888
GCGCGAT16600.016.84590732-33
CCGCCAT45700.016.542881
CGCGATT22150.016.51984632-33
GCGCGAC2001.5693877E-716.36203424-25
CGAGACG10300.016.1824553
AGGCGCG17150.016.13219330-31
CGCGCAT4503.4369805E-815.8742161
Produced by FastQC (version 0.11.5)