FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004982554

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982554
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31938401
Sequences flagged as poor quality0
Sequence length125
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA496390.1554210556752669No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG114350.055.034335
ACCGCGT119700.052.5745666
CCGCGTT129200.048.7548267
AGACCGC131900.047.6223454
CAGACCG133400.047.578023
CGCGTTC137000.045.935598
GCGGGTT24700.037.5984761
CCGCGCG801.19453136E-437.206831
GCCGCGA2850.035.5096741
CGTCGCG1355.8398655E-835.2775841
GCGTTCT186750.033.82589
CGCGTCG1251.2742585E-633.3373151
CGCGATT6550.032.7192881
CGCGCGT851.2656528E-831.49248922-23
CGCGTTT8300.027.9723631
GCGCGAA7450.027.9675481
CGGGCCT39900.025.0656531
GCGGGAT51600.024.9199221
CGAGTCG5500.024.874218
TCAGACC267000.024.7960412
Produced by FastQC (version 0.11.5)